DOI RECORD
Application of Adsorption Chromatography in the Papain Processing
DOI10.7603/s40837-014-0002-8
PublisherSpringer Science and Business Media LLC
Journal / SourceGSTF Journal of Chemical Sciences
Published2014-07-01
Metadata Deposited2023-08-29 (updated: 2023-08-29)
Subject—
Languageen
ISSN2339-5079
Typejournal-article
Volume / Issue / Pages1 / 2 / —
Citations0
References deposited10
Access / license metadataOpen license identified License 1 ↗A reuse license does not by itself establish whether the full text is freely readable.
Abstract
AbstractA direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa·s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice.