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DOI RECORD

Division tracking, index sorting, and single-cell entropy in human haematopoietic stem cells v1

Jaba Tqemaladze

DOI10.17504/protocols.io.r3l25mejxl1y/v1

PublisherSpringer Science and Business Media LLC

Journal / Source—

Published2026-10-05

Metadata Deposited2026-10-06 (updated: 2026-10-06)

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Typeposted-content

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Citations0

References deposited0

Access / license metadataOpen license identified License 1 ↗A reuse license does not by itself establish whether the full text is freely readable.

Abstract

This protocol produces the primary dataset of the Track A core pilot of the StemEntropy programme: human haematopoietic stem cells (HSCs) with known division history and per-cell age-associated single-cell entropy, from the same cells. Peripheral blood (G-CSF mobilised) or bone-marrow-derived CD34+ HSCs are enriched, labelled with CFSE or PKH26, cultured under division-driving conditions with time-resolved sampling, index-sorted by dye-dilution peak, and processed for single-cell RNA-seq (and optionally scATAC-seq). Division number and effective division rate are computed from dye dilution; age-associated entropy is computed by the fixed pipeline of the Stage 1 report (re-derived HSC ageing-clock component as primary selection metric, VarID2 noise as cross-check, manifold-deviation entropy as exploratory). The dataset decides hypotheses H1 (division–entropy association) and H2 (selectability) of the Registered Report.

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