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DOI RECORD

Staining of RAW cells for RNA oxidation v1

Divyangi Kantak, Yahaira Naaldijk iD, Sabine Hilfiker

DOI10.17504/protocols.io.qpvonx76zl4o/v1

PublisherSpringer Science and Business Media LLC

Journal / Source—

Published2026-10-05

Metadata Deposited2026-10-05 (updated: 2026-10-05)

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Typeposted-content

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Citations0

References deposited0

Access / license metadataOpen license identified License 1 ↗A reuse license does not by itself establish whether the full text is freely readable.

Abstract

Validation of RNA oxidation antibody in cultured RAW cells A. Treatment with RNAse or DNAse B. Isotype control or no primary antibody C. Preabsorption of antibody with nucleotide D. In vitro oxidation prior to staining (this does not notably increase signal)

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