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Validation of RNA oxidation antibody in striatal rat slices v1

Yahaira Naaldijk iD, Divyangi Kantak Kantak, Sabine Hilfiker

DOI10.17504/protocols.io.p2l64kr51vqe/v1
PublisherSpringer Science and Business Media LLC
Journal / Source—
Published2026-10-05
Metadata Deposited2026-10-05 (updated: 2026-10-05)
Subject—
Language—
ISSN—
Typeposted-content
Volume / Issue / Pages— / — / —
Citations0
References deposited0
Access / license metadataOpen license identified License 1 ↗A reuse license does not by itself establish whether the full text is freely readable.

Abstract

Validation of RNA oxidation antibody in striatal rat slices A. Treatment with RNAse or DNAse B. Isotype control or no primary antibody C. Preabsorption of antibody with nucleotide D. In vitro oxidation prior to staining (this does not increase signal)