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DOI RECORD

Lymphocyte Activation and Proliferation Assay v1

Luyanda Hlongwa, Elizabeth Mayne

DOI10.17504/protocols.io.p2l64kmr5vqe/v1

PublisherSpringer Science and Business Media LLC

Journal / Source—

Published2026-10-11

Metadata Deposited2026-10-11 (updated: 2026-10-11)

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Language—

ISSN—

Typeposted-content

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Citations0

References deposited0

Access / license metadataOpen license identified License 1 ↗A reuse license does not by itself establish whether the full text is freely readable.

Abstract

This assay is a lymphocyte activation and proliferation assay that stimulates peripheral blood mononuclear cells (PBMCs) with PWM and ConA. PWM stimulates B and T lymphocytes, while ConA stimulates T lymphocytes. To assess activation of lymphocytes, the upregulation of CD69, CD40, CD80, CD86, CD40L (CD154), and CD134 is assessed. When lymphocytes are stimulated by mitogens, they undergo proliferation. Proliferating lymphoblasts are identified in flow cytometry as they appear higher on the forward and side scatter because of their increased size and complexity.

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