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DOI RECORD

Multiplex PCR-based method for whole-genome sequencing of measles virus from wastewater v2

Sipho Gwala, Joshua Levy iD, Victor Mabasa, Joshua Quick, Chris Kent, Maryam Ahmadi Jeshvaghane, Natasha Singh, Kristian Andersen, Kerrigan McCarthy, Mukhlid Yousif

DOI10.17504/protocols.io.n92ld41wol5b/v2

PublisherSpringer Science and Business Media LLC

Journal / Source—

Published2026-04-14

Metadata Deposited2026-10-09 (updated: 2026-10-09)

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Typeposted-content

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Citations0

References deposited0

Access / license metadataOpen license identified License 1 ↗A reuse license does not by itself establish whether the full text is freely readable.

Abstract

The protocol relies on multiplex PCR to generate overlapping amplicons spanning the full measles virus genome, enabling efficient enrichment of MeV genetic material from low-input and degraded RNA. Amplified products are compatible with Illumina sequencing workflows and support high genome coverage suitable for molecular epidemiology and phylogenetic analysis. This protocol has been optimized for sensitivity and reproducibility using wastewater samples and can be completed within 1-2 days.

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