RESEARCH. CONNECTED.

Explore the scholarly record.

Discover prefix ownership, publishers, journals and DOI metadata. Understand the data behind every publication.

DOI RECORD

NOMADS-IR : Insecticide Resistance Panel for Surveillance v1

Cynthia Awuor Odhiambo, Karolina Mosler, Eric Ochomo, Jason Alexander Hendry, Daniel Bridges iD, Mulenga Mwenda

DOI10.17504/protocols.io.81wgbmx8ovpk/v1

PublisherSpringer Science and Business Media LLC

Journal / Source—

Published2026-08-01

Metadata Deposited2026-10-07 (updated: 2026-10-07)

Subject—

Language—

ISSN—

Typeposted-content

Volume / Issue / Pages— / — / —

Citations0

References deposited0

Access / license metadataOpen license identified License 1 ↗A reuse license does not by itself establish whether the full text is freely readable.

Abstract

The NOMADS- IR panel approach enables genomic surveillance of insecticide resistance markers in Anopheles gambiae mosquitoes using targeted nanopore sequencing. It includes amplicons targeting key insecticide resistance SNPs along targeted regions i.e. acetylcholinesterase 1(Ace1), Glutathione S-transferase epsilon 2 (Gste2), gamma-aminobutyric acid (Gaba)-gated chloride channel and voltage-gated sodium channel (Vgsc). The protocol can be done in 2 days: Day 1 - Multiplex PCR, POST-PCR DNA Clean Up, and Post-PCR DNA Quantification with Qubit steps Day 2 - End Prep and Native Sample Barcoding, Rapid Adapter Ligation and Size selection Cleanup, and Priming and loading the SpotON flow cell

Open DOI