RESEARCH. CONNECTED.

Explore the scholarly record.

Discover prefix ownership, publishers, journals and DOI metadata. Understand the data behind every publication.

DOI RECORD

Rapid and robust cloning of sgRNA expression plasmids v2

Ghanem El Kassem, Michael Boettcher iD

DOI10.17504/protocols.io.4r3l229ojl1y/v2

PublisherSpringer Science and Business Media LLC

Journal / Source—

Published2026-10-08

Metadata Deposited2026-10-09 (updated: 2026-10-09)

Subject—

Language—

ISSN—

Typeposted-content

Volume / Issue / Pages— / — / —

Citations0

References deposited0

Access / license metadataOpen license identified License 1 ↗A reuse license does not by itself establish whether the full text is freely readable.

Abstract

In this lab protocol, we will outline a step-by-step procedure for cloning of spCas9 sgRNAs using oligo annealing and T4 ligation. This protocol will guide you through the crucial steps of designing, annealing, ligating oligonucleotides and digested plasmids, as well as heat shock transformation procedure to clone sgRNAs expression vectors.

Open DOI